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Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-appendix tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of Rat-brain tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) MCF7, 2) Rat Kidney Tissue, 3) Mouse Brain Tissue, diluted at 1:2000. |
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IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100. |
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