Catalog_noA1236
Product_nameHistone H3 Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p
ReactivityHuman,Mouse,Rat
Size50μl/100μl
Price580.00/880.00
immunogenSynthetic Peptide of Histone H3
SpecificityThe antibody detects endogenous Histone H3 protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceRabbit
DilutionWB: 1:500-1000 IHC: 1:200-500
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Observed_band15-17KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
BackgroundHistone H3 is one of the five main histone proteins involved in the structure of chromatin ineukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limitingDNA accessibility to the cellular machineries whic
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    Western blot analysis of 1) Hela, 2) 3T3 using Histone H3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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