Catalog_noA1055
Product_nameHistone H3 Monoclonal Antibody(1G1)
Category抗原抗体
ApplicationsWB,IHC-p,IF,IP
ReactivityHuman,Mouse,Rat,Yeast
Size50μl/100μl
Price580.00/880.00
immunogenRecombinant Protein of Histone H3
SpecificityThe antibody detects endogenous Histone H3 protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:2000-5000 IF:1:100-500 IP:1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Observed_band15KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
BackgroundHistone H3 is one of the five main histone proteins involved in the structure of chromatin ineukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limitingDNA accessibility to the cellular machineries whic
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    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H3 Monoclonal Antibody(1G1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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