博奥龙Logo
快速订购 快速订购: English 中文版
Catalog_noBD-PD400141
Product_nameMERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Protein (aa 1-725) Insect Cell Lysate (WB positive control)
Category冠状病毒产品
注意事项1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
ApplicationsWestern Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Size300μg
Price2340
SourceBaculovirus-Insect cells
Storage_stabilityStore at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
稀释液/缓冲液1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Observed_bandThe recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
BackgroundThe spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
裂解缓冲液Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Instructions 查看与下载
Share


  • 产品详情
  • 相关产品
  • 用户反馈
  • 发表文献
  • 在线问答
    This MERS-CoV CoV spike overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CoV spike protein (Cat: 40069-V08B1) from the overexpression lysate was verified.
联系我们
北京博奥龙免疫技术有限公司
地址:苏州相城经济开发区观塘路1号西交大漕湖科技园C幢810室
电话:400-921-8278
联系人:刘经理
手机:13552826508 / 13552713240
北京博奥龙微信公众号
扫一扫关注我们

Copyright © 2021 苏州博特龙免疫技术有限公司 版权所有 ,苏ICP备2021013855号-1  

佐剂 细胞冻存液 抗体制备 慢病毒包装 免疫检测