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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of Mouse-brain tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of Rat-brain tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200. |
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Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200. |
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Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000. |
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