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Catalog_no	AM3519
Product_name	α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)
Category	抗原抗体
Applications	WB,IF,IHC-p
Reactivity	Human,Rat,Mouse
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	TUBA1B
Protein_name	Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)
Human swiss prot no	7277
Human swiss prot no	P68363
immunogen	Synthetic Peptide of α-tubulin (Acetyl Lys40)
Specificity	α-tubulin (Acetyl Lys40) protein detects endogenous levels of α-tubulin (Acetyl Lys40)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Mouse
Dilution	WB 1:1000-2000, IHC 1:50-100 IF 1:200
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
othername	Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)
Observed_band	52KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Human-liver-cancer tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Mouse-lung tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Rat-spinal-cord tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl- a-tubulin(Lys40) Mouse mAb 1:2000.
	Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.
	Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.

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