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Catalog_no	AM3136
Product_name	eIF4A1 Monoclonal Antibody(M8)
Category	抗原抗体
Applications	WB,IHC-p,IF
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	EIF4A1
Protein_name	Eukaryotic initiation factor 4A-I
Human swiss prot no	1973
Human swiss prot no	P60842
Mouse gene id	13681
Mouse swiss prot no	P60843
Rat swiss prot no
immunogen	Synthetic Peptide of eIF4A1
Specificity	The antibody detects endogenous eIF4A1 protein.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:1000-3000 IF: 1:100-200 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1)
Observed_band	48KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue,
	IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.