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Catalog_no	AM3123
Product_name	Collagen III Monoclonal Antibody(Q76)
Category	抗原抗体
Applications	WB,IHC-p,IF,
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	COL3A1
Protein_name	Collagen alpha-1(III) chain
Human swiss prot no	1281
Human swiss prot no	P02461
Mouse gene id	12825
Mouse swiss prot no	P08121
Rat gene id	84032
Rat swiss prot no	P13941
immunogen	Synthetic Peptide of Collagen III
Specificity	The antibody detects endogenous Collagen III protein.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:1000 IF 1:200 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	COL3A1; Collagen alpha-1(III) chain
Observed_band	138KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Mouse-liver tissue. 1,Collagen III Monoclonal Antibody(Q76)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of Hela, diluted at 1:1000.