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Catalog_no	AM3091
Product_name	Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3)
Category	抗原抗体
Applications	WB,IHC-p,IF,IP
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B
Protein_name	Histone H3.1/Histone H3.2/Histone H3.3
Human swiss prot no	8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Human swiss prot no	P68431/Q71DI3/P84243
Rat gene id	291159/100361558
Rat swiss prot no	Q6LED0/P84245
immunogen	Synthetic Peptide of Histone H3 (Tri Methyl Lys79)
Specificity	The antibody detects endogenous Histone H3 (tri methyl K79) protein.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Observed_band	15KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Human-appendix tissue. 1,Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Rat-liver tissue. 1,Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
	1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

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