| Catalog_no | AM3089 |
| Product_name | Oct1 Monoclonal Antibody(7G1) |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF, |
| Reactivity | Human |
| Size | 100μg/50μg |
| Price | 2000.00/1100.00 |
| Gene_name | POU2F1 |
| Protein_name | POU domain class 2 transcription factor |
| Human swiss prot no | 5451/5452 |
| Human swiss prot no | P14859/P09086 |
| Rat gene id | 171068 |
| Rat swiss prot no | P31503 |
| immunogen | Synthetic Peptide of 42644 |
| Specificity | The antibody detects endogenous Oct1 protein. |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse |
| Dilution | WB: 1:500-2000 IF 1:200 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| Observed_band | 89KD |
| Instructions | 
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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
