Catalog_noAM3041
Product_nameBcl-2 Monoclonal Antibody(6B5)
Category抗原抗体
ApplicationsIF,WB,IHC-p
ReactivityHuman,Mouse,Rat,chicken
Size100μg/50μg
Price2000.00/1100.00
Gene_nameBCL2
Protein_nameApoptosis regulator Bcl-2
Human swiss prot no596
Human swiss prot noP10415
Mouse gene id12043
Mouse swiss prot noP10417
Rat gene id24224
Rat swiss prot noP49950
immunogenSynthetic Peptide of Bcl-2
SpecificityThe antibody detects endogenous Bcl-2 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionIF: 1:50-200 WB: 1:1000~2000 IHC: 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameBCL2; Apoptosis regulator Bcl-2
Observed_band26KD
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    Western blot analysis of Hela, diluted at 1:1000
    IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
    The picture was kindly provided by our customer
    Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
    Western blot detection of Bcl-2 in human breast cancer cell line MCF-7(A), MDA-MB-231(B) and Cal51(C) using Bcl-2 mouse mAb (1:2000 diluted).Predicted band size: 26kDa.Observed band size:26kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bcl-2 Mouse mAb diluted at 1:200 (4°,overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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